R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GLIPR2 (F7H7V) Rabbit mAb #86955
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GLIPR2 (F7H7V) Rabbit mAb recognizes endogenous levels of total GLIPR2 protein. An unknown background band is detected at 65 kDa in some human and mouse cell lines.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro135 of human GLIPR2 protein.
Background
Glioma pathogenesis-related protein 2 (GLIPR2), also known as Golgi-associated PR-1 protein (GAPR-1), is a Golgi-associated protein belonging to the PR-1 protein family (1). GLIPR2 contains a sperm-coating protein (SCP) domain, also known as a CAP domain; however, in contrast to other SCP domain-containing proteins, it does not have a signal peptide and is not secreted via the classical secretory pathway (2). GLIPR2 is expressed in monocytes, macrophages, natural killer (NK) cells, and T cell subsets (3). GLIPR2 binds Beclin-1 and is a negative regulator of autophagy that inhibits PtdIns3K-C1 complex activity (4,5). GLIPR2 promotes epithelial to mesenchymal transition in vitro, and is expressed at high levels in fibrotic kidney (6). GLIPR2 has been identified as a marker for type 1 migratory dendritic cells (DC1s) (7) and a potential biomarker and tumor suppressor in a pan-cancer analysis (3).
- Eberle, H.B. et al. (2002) J Cell Sci 115, 827-38.
- Serrano, R.L. et al. (2004) J Mol Biol 339, 173-83.
- Lin, W. et al. (2024) Front Immunol 15, 1280525.
- Shoji-Kawata, S. et al. (2013) Nature 494, 201-6.
- Zhao, Y. et al. (2021) Autophagy 17, 2891-2904.
- Baxter, R.M. et al. (2007) Matrix Biol 26, 20-9.
- Ngoi, S. et al. (2022) J Immunol 209, 1200-1211.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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