Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
GBP1 (F4Y1Y) Rabbit mAb #32671
Filter:
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
GBP1 (F4Y1Y) Rabbit mAb recognizes endogenous levels of total GBP1 protein. This antibody does not cross-react with GBP2 or GBP3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GBP1 protein.
Background
Guanylate-binding proteins (GBPs) are a family of dynamin-related proteins that facilitate host defense against intracellular bacterial and viral pathogens (1). Interferon (IFN) signaling upregulates the expression of several GBPs in response to infection, including the most extensively studied paralog GBP1 (1). GBP1 specifically binds to lipopolysaccharide (LPS) on the cell surface of cytosolic Gram-negative bacteria, forming a stable protein layer and disrupting the integrity of the outer membrane (2). GBP1 also recruits caspase-4 to the membrane and promotes the pro-inflammatory cell death program known as pyroptosis (3,4). GBP1 is increasingly studied in the context of cancer, where it appears to restrict cellular proliferation in breast and colorectal cancers but may promote growth and chemoresistance in cases of ovarian cancer and glioblastoma (5-9). Elevated GBP1 expression levels also correlate with enhanced immune cell infiltration and increased overall survival in breast cancer patients treated with immunotherapy (10).
- Kutsch, M. and Coers, J. (2021) FEBS J 288, 5826-5849.
- Kutsch, M. et al. (2020) EMBO J 39, e104926.
- Wandel, M.P. et al. (2020) Nat Immunol 21, 880-891.
- Dickinson, M.S. et al. (2023) Proc Natl Acad Sci USA 120, e2216028120.
- Honkala, A.T. et al. (2019) Front Immunol 10, 3139.
- Ascierto, M.L. et al. (2012) Breast Cancer Res Treat 131, 871-80.
- Britzen-Laurent, N. et al. (2013) Carcinogenesis 34, 153-62.
- De Donato, M. et al. (2012) J Cell Physiol 227, 1034-41.
- Lan, Q. et al. (2016) Oncotarget 7, 9680-91.
- Zhao, Y. et al. (2022) Front Genet 13, 820135.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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