R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Gab3 (F3W6T) Rabbit mAb #34612
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70, 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Gab3 (F3W6T) Rabbit mAb recognizes endogenous levels of total Gab3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu481 of human Gab3 protein.
Background
Grb2-associated-binding protein 3 (Gab3) is a member of the Grb-associated binder (Gab) family of adaptor proteins that support the assembly of activated signaling complexes through docking and scaffolding functions (1). The Gab family proteins, Gab1-3, contain a conserved N-terminal pleckstrin homology (PH) domain and multiple phosphorylation sites specific for tyrosine kinases (2). The PH-domain interacts with lipids like phosphatidylinositol-3,4,5-triphosphate (PIP3) to mediate membrane recruitment, and the phosphorylated tyrosines act as recruitment sites for SH2 domain-containing proteins (3,4). Gab3 is expressed in hematopoietic lineage cells, including natural killer (NK) cells, mast cells, and memory CD8+ T cells (5). Gab3 plays an important role in peripheral NK cell expansion and is essential for IL-2/IL-15 receptor-mediated MAPK signaling in NK cells (6). Gab3 expression is associated with shorter progression-free survival in ovarian cancer and may be a promising biomarker to predict immunotherapeutic responses in lung adenocarcinoma (LUAD) and melanoma (7-9).
- Liu, Y. and Rohrschneider, L.R. (2002) FEBS Lett 515, 1-7.
- Nishida, K. and Hirano, T. (2003) Cancer Sci 94, 1029-33.
- Lemmon, M.A. and Ferguson, K.M. (2001) Biochem Soc Trans 29, 377-84.
- Nakaoka, Y. and Komuro, I. (2013) Int J Inflam 2013, 141068.
- Seiffert, M. et al. (2003) Mol Cell Biol 23, 2415-24.
- Sliz, A. et al. (2019) Sci Immunol 4, eaav3866. doi: 10.1126/sciimmunol.aav3866.
- Berkel, C. and Cacan, E. (2021) J Cell Commun Signal 15, 57-70.
- Wang, N. et al. (2022) J Biochem Mol Toxicol 36, e23166.
- Li, C. et al. (2023) Melanoma Res 33, 27-37.
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