Folate Receptor Alpha/FOLR1 (E8U2F) Mouse mAb (BSA and Azide Free) #30124
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38 |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
This product is the carrier free version of product #34265. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #34265
For standard formulation of this product see product #34265
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Folate Receptor Alpha/FOLR1 (E8U2F) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total Folate Receptor Alpha/FOLR1 protein. Non-specific staining was observed in normal human brain by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp77 of human Folate Receptor Alpha/FOLR1 protein.
Background
Folate Receptor Alpha (FOLR1) is a cysteine-rich cell-surface receptor that plays a critical role in the maintenance and transport of folate (vitamin B9) and reduced folic acid derivatives into cells and is essential for various cellular processes (1,2). FOLR1 binds to folate and facilitates its internalization into cells through receptor-mediated endocytosis or potocytosis and is important in cellular processes, including DNA synthesis and repair (1-3). Intracellular folate levels are critical to maintaining and supporting folate functions in its multiple coenzyme forms (4). It is primarily expressed in tissues requiring high folate levels, such as the placenta, kidneys, and certain cancer cells (5). FOLR1 has been found to be overexpressed in several types of cancer, including ovarian, lung, and breast cancer (6-8).
- Chen, C. et al. (2013) Nature 500, 486-9.
- Wibowo, A.S. et al. (2013) Proc Natl Acad Sci USA 110, 15180-8.
- Orr, R.B. and Kamen, B.A. (1994) Cancer Res 54, 3905-11.
- Bailey, L.B. and Gregory, J.F. (1999) J Nutr 129, 779-82.
- Lacey, S.W. et al. (1989) J Clin Invest 84, 715-20.
- Bax, H.J. et al. (2023) Br J Cancer 128, 342-353.
- Nunez, M.I. et al. (2012) J Thorac Oncol 7, 833-40.
- Necela, B.M. et al. (2015) PLoS One 10, e0122209.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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