R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
FIZZ1 (E9O2P) Rabbit mAb #29744
Filter:
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
FIZZ1 (E9O2P) Rabbit mAb recognizes endogenous levels of total mouse FIZZ1 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro42 of mouse FIZZ1 protein.
Background
Found in inflammatory zone 1 (FIZZ1) protein, also known as resistin-like molecule α (RELMα) and hypoxia-induced mitogenic factor (HIMF), is a secreted protein with a cysteine-rich carboxyl-terminus (1). FIZZ1 is a member of the RELM family of proteins which consists of four isoforms in mice and two isoforms in humans. The murine family members are FIZZ1, FIZZ2, FIZZ3, and FIZZ4 and the human proteins are Resistin and RELMβ (2). FIZZ1 is expressed in lung, bone marrow, and spleen tissue (1,3). FIZZ1 transcription is mediated by a T helper type 2 (Th2) cytokine environment and its upregulation has been observed in inflammatory and infectious environments, including allergic airway inflammation, colitis, and helminth infection (4,5). FIZZ1 has been shown to drive myeloid cell chemotaxis and to downregulate Th2 cytokine production, both in a Bruton’s tyrosine kinase (BTK)-dependent manner (6,7). FIZZ1 is induced in a mouse chronic hypoxia model of pulmonary hypertension and activates the PI3K/Akt pathway, driving proliferation of pulmonary microvascular smooth muscle cells (8). FIZZ1 has been identified as a marker for alternatively activated macrophages, also known as M2 macrophages, which are characterized as anti-inflammatory and are hallmarks of several disease states, including infection, allergy, diabetes, and cancer (7,9,10).
- Holcomb, I.N. et al. (2000) EMBO J 19, 4046-55.
- Fan, C. et al. (2013) Histochem Cell Biol 139, 605-13.
- Liu, T. et al. (2004) J Immunol 173, 3425-31.
- Stütz, A.M. et al. (2003) J Immunol 170, 1789-96.
- Osborne, L.C. et al. (2013) J Immunol 190, 2292-300.
- Su, Q. et al. (2007) FASEB J 21, 1376-82.
- Nair, M.G. et al. (2009) J Exp Med 206, 937-52.
- Teng, X. et al. (2003) Circ Res 92, 1065-7.
- Nair, M.G. et al. (2003) Immunol Lett 85, 173-80.
- Jablonski, K.A. et al. (2015) PLoS One 10, e0145342.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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