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  3. Exportin-1/CRM1 (D6V7N) Rabbit mAb (BSA and Azide Free)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Exportin-1/CRM1 (D6V7N) Rabbit mAb (BSA and Azide Free) #49000

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    Supporting Data

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa) 123
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #46249. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #46249

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Exportin-1/CRM1 (D6V7N) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total exportin-1/CRM1 protein.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human exportin-1/CRM1 protein.

    Background

    Exportins are a family of seven proteins that are responsible for intracellular transport. Exportin-1, also known as chromosome region maintenance 1 (CRM1), is a protein essential for nuclear export of hundreds of proteins, mRNAs, and rRNAs (1-3). Exportin-1 binds to substrates with nuclear export signals (NESs) rich in leucine and other hydrophobic amino acids (4). These hydrophobic sequences form an alpha-helix-loop that can bind to the exportin-1 hydrophobic groove (5). Studies have shown that these NESs can be modified either by protein modifications or by mutation to regulate exportin-1 binding (6-7). Targets of exportin-1 include many tumor suppressors, such as Rb, p53, FoxO1, BAF47, as well as oncoproteins, such as p21 and p27 (1). In addition, Myc can upregulate exportin-1 during biogenesis, where it can export newly formed 40S and 60S subunits from the nucleoli (8-9).

    Inhibition of nuclear export has been pursued for therapeutic application since the finding that leptomycin B could suppress HIV replication by suppressing the ability of exportin-1 to export the HIV-1 protein Rev (2, 10). Overexpression of exportin-1 has been associated with poor prognosis in various cancer types (11-13). Genomic approaches and development of inhibitors have identified exportin-1 as a druggable target (14-16). The use of various inhibitors of exportin-1 is also being explored in various antiviral therapies (17-18).
    1. Xu, D. et al. (2012) Mol Biol Cell 23, 3673-6.
    2. Ossareh-Nazari, B. et al. (1997) Science 278, 141-4.
    3. Fukuda, M. et al. (1997) Nature 390, 308-11.
    4. Kutay, U. and Güttinger, S. (2005) Trends Cell Biol 15, 121-4.
    5. Dong, X. et al. (2009) Nat Struct Mol Biol 16, 558-60.
    6. Vogt, P.K. et al. (2005) Cell Cycle 4, 908-13.
    7. Craig, E. et al. (2002) EMBO J 21, 31-42.
    8. Thomas, F. and Kutay, U. (2003) J Cell Sci 116, 2409-19.
    9. Golomb, L. et al. (2012) Mol Cell 45, 222-32.
    10. Wolff, B. et al. (1997) Chem Biol 4, 139-47.
    11. Noske, A. et al. (2008) Cancer 112, 1733-43.
    12. Shen, A. et al. (2009) Neurosurgery 65, 153-9; discussion 159-60.
    13. Yao, Y. et al. (2009) Oncol Rep 21, 229-35.
    14. Schmidt, J. et al. (2013) Leukemia 27, 2357-65.
    15. Boyle, S.M. et al. (1999) J Virol 73, 6872-81.
    16. Walker, C.J. et al. (2013) Blood 122, 3034-44.
    17. Yadav, V. et al. (2016) Virusdisease 27, 357-368.
    18. Chutiwitoonchai, N. et al. (2017) Virology 507, 32-39.

    Database Links

    UniProt ID: O14980


    Entrez-Gene Id: 7514


    限制使用

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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