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Estrogen Receptor alpha (D8H8) Rabbit Monoclonal Antibody

Estrogen Receptor alpha (D8H8) Rabbit Monoclonal Antibody #8644

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IP
ChIP
C&R

Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	66
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
ChIP-Chromatin Immunoprecipitation
C&R-CUT & RUN

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
Chromatin IP	1:100
Chromatin IP-seq	1:100
CUT&RUN	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #36911.

Protocol

Specificity / Sensitivity

Estrogen Receptor alpha (D8H8) Rabbit Monoclonal Antibody recognizes endogenous levels of total ERα protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val560 of human ERα protein.

Background

Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).

Alternate Names

DKFZp686N23123; E2 receptor alpha; ER; ER-alpha; Era; ESR; ESR1; ESRA; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor alpha delta 3; estrogen receptor alpha delta 4; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha

Database Links

UniProt ID: P03372

Entrez-Gene Id: 2099

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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