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  3. Drosha (D30F3) Rabbit mAb
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Drosha (D30F3) Rabbit mAb #3410

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  • WB
  • ChIP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 160
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:25

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Drosha (D30F3) Rabbit mAb detects endogenous levels of total Drosha protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His549 of human Drosha.

    Background

    Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3).

    Alternate Names

    drosha; ETOHI2; HSA242976; nuclear RNase III Drosha; p241; RANSE3L; Ribonuclease; Ribonuclease 3; Ribonuclease III; ribonuclease III RNASEN; ribonuclease type III; RN3; RNA SD; RNase; RNase III; RNASEN

    Database Links

    UniProt ID: Q9NRR4


    Entrez-Gene Id: 29102


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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