Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DNA Polymerase β (F5I2R) Rabbit mAb #89011
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DNA Polymerase β (F5I2R) Rabbit mAb recognizes endogenous levels of total DNA polymerase β protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn245 of human DNA polymerase β protein.
Background
DNA polymerase β (POLB) functions in the base excision repair (BER) DNA repair pathway, repairing single-strand DNA breaks (SSBs) arising from oxidative damage, deamination, alkylation, or abasic sites (1). In mitochondria, oxidative stress causes recruitment of DNA polymerase β to repair damaged mitochondrial DNA (mtDNA) (2).
DNA polymerase β expression levels and mutations are associated with human cancer (3-6). Inhibition or downregulation of DNA polymerase β is synthetic lethal with defective mismatch repair (MMR) in models of acute lymphoblastic leukemia (ALL) (7) and with BRCA1 deficiency in models of breast cancer (8).
DNA polymerase β expression levels and mutations are associated with human cancer (3-6). Inhibition or downregulation of DNA polymerase β is synthetic lethal with defective mismatch repair (MMR) in models of acute lymphoblastic leukemia (ALL) (7) and with BRCA1 deficiency in models of breast cancer (8).
- Gohil, D. et al. (2023) Int J Mol Sci 24, 14186. doi: 10.3390/ijms241814186.
- Horton, J.K. et al. (2022) DNA Repair (Amst) 116, 103357.
- Shahi, A. and Kidane, D. (2024) Clin Exp Med 24, 239.
- Al-Kawaz, A. et al. (2021) Breast Cancer Res Treat 190, 39-51.
- Qin, J. et al. (2021) Neoplasia 23, 663-675.
- Kladova, O.A. et al. (2022) Int J Mol Sci 23, 2390. doi: 10.3390/ijms23042390.
- Teng, J.Y. et al. (2023) Leukemia 37, 1204-1215.
- Yuhas, S.C. et al. (2021) ACS Chem Biol 16, 1339-1343.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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