Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Cyclophilin B (D1V5J) Rabbit mAb #43603
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Cyclophilin B (D1V5J) Rabbit mAb recognizes endogenous levels of total cyclophilin B protein. This antibody does not cross-react with cyclophilin A protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human cyclophilin B protein.
Background
Cyclophilin B (CyPB) is an ER-localized chaperone protein belonging to the family of peptidyl-prolyl cis-trans isomerases (PPIases) (1,2). Research studies have demonstrated that CyPB associates with type I procollagen and is involved in its sorting and transport through the secretory compartment (3). Mutations in the gene encoding CyPB, PPIB, lead to aberrant biosynthesis of type I procollagen, which underlies the pathogenesis of osteogenesis imperfecta (OI), a disorder characterized by bone fragility (4-7). In additional to its role in OI, research studies demonstrate that CyPB overexpression supports the expression of multiple oncogenic drivers of glioblastoma multiforme (8).
- Price, E.R. et al. (1991) Proc Natl Acad Sci U S A 88, 1903-7.
- Hasel, K.W. et al. (1991) Mol Cell Biol 11, 3484-91.
- Smith, T. et al. (1995) J Biol Chem 270, 18323-8.
- Pyott, S.M. et al. (2011) Hum Mol Genet 20, 1595-609.
- van Dijk, F.S. et al. (2009) Am J Hum Genet 85, 521-7.
- Cabral, W.A. et al. (2014) PLoS Genet 10, e1004465.
- Choi, J.W. et al. (2009) PLoS Genet 5, e1000750.
- Choi, J.W. et al. (2014) Cancer Res 74, 484-96.
Alternate Names
cyclophilin B; CYP-S1; CyPB; peptidyl prolyl isomerases; PPIB; PPIs; S-cyclophilin; SCYLP
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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