Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CRTAM (F5I5L) Rabbit mAb #30871
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CRTAM (F5I5L) Rabbit mAb recognizes endogenous levels of total CRTAM protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CRTAM protein.
Background
Class I-restricted T cell-associated molecule (CRTAM) is a type I transmembrane glycoprotein that plays a role in T cell development, proliferation, and adhesion (1). CRTAM is transiently expressed on the surface of activated CD8+ T cells and natural killer (NK) cells, where it binds to its ligand CADM1 (2,3). CRTAM is also expressed on particular subsets of activated CD4+ T cells and is required for the differentiation of CD4+ cytotoxic T cells (CTLs) (4-6). CRTAM binding to CADM1 has been shown to enhance the adhesive capacity and cytotoxicity of CD8+ T cells and NK cells (2). It also promotes retention and proliferation of CTLs in draining lymph nodes via interaction with CADM1-expressing dendritic cells (DCs) (7). Interestingly, elevated CRTAM expression has been found to be associated with increased survival in patients with triple negative breast cancer (TNBC), suggesting its use as a potential biomarker for immunotherapy efficacy (8).
- Hermans, D. et al. (2023) Biology (Basel) 12, 452. doi: 10.3390/biology12030452.
- Arase, N. et al. (2005) Int Immunol 17, 1227-37.
- Barragan-Galvez, J.C. et al. (2024) Biochem Biophys Rep 39, 101813.
- Yeh, J.H. et al. (2008) Cell 132, 846-59.
- Takeuchi, A. and Saito, T. (2017) Front Immunol 8, 194.
- Takeuchi, A. et al. (2016) J Exp Med 213, 123-38.
- Takeuchi, A. et al. (2009) J Immunol 183, 4220-8.
- Zheng, S. et al. (2024) Int Immunopharmacol 129, 111625.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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