Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CREB (48H2) Feline Chimeric mAb #47692
Filter:
- IF
Supporting Data
| REACTIVITY | H M R Mk Dm |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Feline chimera IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Dm-D. melanogaster
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as CREB (48H2) Rabbit mAb #9197.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CREB (48H2) Feline Chimeric mAb recognizes endogenous levels of total CREB-1 protein. This antibody does not cross-react with other ATF/CREB family members. Non-specific staining of components along the retinotectal pathway was observed by immunofluorescence in fixed frozen mouse tissue. Non-specific signal is observed in formaldehyde fixed frozen mouse retina by immunofluorescence.
Species Reactivity:
Human, Mouse, Rat, Monkey, D. melanogaster
Source / Purification
This recombinant chimeric antibody is engineered from CREB (48H2) Rabbit mAb #9197 according to animal-free protocols. This chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, CREB (48H2) Rabbit mAb #9197, is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CREB-1 protein. The epitope has been mapped to residues surrounding Glu21.
The parent antibody, CREB (48H2) Rabbit mAb #9197, is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CREB-1 protein. The epitope has been mapped to residues surrounding Glu21.
Background
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
限制使用
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