CDYL (E7S8T) Rabbit mAb #18764

Chromodomain Y-like (CDYL) is a transcriptional corepressor and chromatin reader that recognizes the histone marks H3K9me3, H3K27me2, and H3K27me3, which are typically associated with repressive chromatin states and inhibition of gene expression. CDYL functions as a molecular bridge between the histone H3K9 methyltransferase G9a/EHMT2 and the transcription repressor REST, targeting G9a to the loci of REST target genes (1,2). CDYL has also been shown to directly interact with EZH2, the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2), where it recruits EZH2 to H3K27me3 sites and propagates this repressive modification along chromatin (3). CDYL binds to methylated histone lysine residues through its N-terminal chromodomain, while its C-terminal enoyl-coenzyme A (CoA) hydratase-isomerase catalytic domain may be involved in histone lysine crotonylation (4). Knockout of CDYL is largely embryonic lethal in mice, with few mice surviving and dying shortly after birth (5). Additional studies in mice show that knockdown of CDYL appears to disrupt normal neuronal migration and brain development, possibly due to the loss of RhoA transcriptional repression (6).