Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD68 (E3O7V) Feline Chimeric mAb #83676
Filter:
- IF
Supporting Data
| REACTIVITY | M R Hm |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Feline Chimera IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
- Hm-Hamster
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as CD68 (E3O7V) Rabbit mAb #97778.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD68 (E3O7V) Feline Chimeric mAb recognizes endogenous levels of total CD68 protein.
Species Reactivity:
Mouse, Rat, Hamster
Source / Purification
This recombinant chimeric antibody is engineered from CD68 (E3O7V) Rabbit mAb #97778 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, CD68 (E3O7V) Rabbit mAb #97778, is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.
The parent antibody, CD68 (E3O7V) Rabbit mAb #97778, is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.
Background
CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).
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For Research Use Only. Not for Use in Diagnostic Procedures.
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