CD44 (IM7) Rat mAb #39037
Filter:
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. This product is stable for 60 months when stored at -20C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD44 (IM7) Rat mAb recognizes endogenous levels of total CD44 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD44. In addition to mouse reactivity, this clone has shown to be positive for human samples by Flow.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.
Background
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin, and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).
- Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
- Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
- Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
- Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
- Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
- Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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