R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CCR4 (F9A9K) Rabbit mAb #59945
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CCR4 (F9A9K) Rabbit mAb recognizes endogenous levels of total CCR4 protein. This antibody detects a 15-20 kDa protein of unknown identity in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCR4 protein.
Background
C-C chemokine receptor type 4 (CCR4) is a chemokine receptor that is primarily expressed in T cells, specifically in Th2, Th17, and Treg subsets (1). Like other chemokine receptors, CCR4 is a G protein-coupled receptor (GPCR) that contains seven transmembrane domains (2). CCR4 selectively binds with the chemokine ligands CCL17, also known as thymus and activation-related chemokine (TARC), and CCL22, also known as macrophage-derived chemokine (MDC). CCL17/TARC functions as a chemoattractant for CCR4-expressing T cells and is associated with type 2 immune responses (3,4). CCL22/MDC is expressed by macrophages and dendritic cells and also functions as a chemoattractant with dominant binding to CCR4 compared with CCL17/TARC (5-7). Elevated CCR4 expression has been implicated in various allergic diseases, such as asthma and dermatitis (8,9). It has also been implicated in several hematological malignancies, including acute myeloid leukemia (AML), acute T cell leukemia/lymphoma (ATLL), and cutaneous T cell lymphoma (CTLL) (1,10). The humanized anti-CCR4 monoclonal antibody mogamulizumab has been approved for the treatment of patients with relapsed ATLL (11).
- Yoshie, O. (2021) Cancers (Basel) 13, 5542. doi: 10.3390/cancers13215542.
- Solari, R. and Pease, J.E. (2015) Eur J Pharmacol 763, 169-77.
- Imai, T. et al. (1996) J Biol Chem 271, 21514-21.
- Islam, S.A. and Luster, A.D. (2012) Nat Med 18, 705-15.
- Imai, T. et al. (1998) J Biol Chem 273, 1764-8.
- Mariani, M. et al. (2004) Eur J Immunol 34, 231-40.
- Viney, J.M. et al. (2014) J Immunol 192, 3419-27.
- Perros, F. et al. (2009) Allergy 64, 995-1002.
- Smith, J.S. et al. (2018) Dermatitis 29, 179-186.
- Zou, S. et al. (2024) Discov Oncol 15, 412.
- Ureshino, H. et al. (2019) Clin Lymphoma Myeloma Leuk 19, 326-331.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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