Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Cathepsin A (F8X9E) Rabbit mAb #67452
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 54, 20 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Cathepsin A (F8X9E) Rabbit mAb recognizes endogenous levels of total cathepsin A protein. This antibody detects the full-length (inactive) cathepsin A precursor in addition to the processed (active) light chain. This antibody detects a 75 kDa protein of unknown identity in some cell lines by western blotting. Non-specific nuclear staining was observed in testis by immunohistochemistry.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human cathepsin A protein.
Background
Cathepsin A, also known as lysosomal protective protein for beta-galactosidase (PPGB), functions as both a serine protease and a critical enzyme for stabilizing β-galactosidase and neuraminidase (1,2). Similar to other cathepsin family members, cathepsin A is synthesized as an inactive 54 kDa precursor, then cleaved into catalytically active large (32 kDa) and small (20 kDa) subunits that form a disulfide-linked heterodimer after excision of a linker peptide (1-3). Cathepsin A degradation targets include endothelin (4), LAMP2 (5), and angiotensin I (6). CTSA gene mutations result in protein misfolding and lysosomal galactosialidosis, as neuraminidase and β-galactosidase activity depend on direct association with cathepsin A (7,8). Cathepsin A overexpression in heart causes excess degradation of superoxide dismutase (SOD), increasing oxidative stress, inflammation, and remodeling of the extracellular matrix (ECM) (9). High expression of cathepsin A has been reported and associated with metastasis in melanocytic tumors, colorectal cancer, hepatocellular carcinoma, and lung adenocarcinoma (10-13).
- Galjart, N.J. et al. (1988) Cell 54, 755-64.
- Bonten, E.J. et al. (1995) J Biol Chem 270, 26441-5.
- Kolli, N. and Garman, S.C. (2014) J Biol Chem 289, 11592-11600.
- Itoh, K. et al. (1995) J Biol Chem 270, 515-8.
- Cuervo, A.M. et al. (2003) EMBO J 22, 47-59.
- Jackman, H.L. et al. (2002) Hypertension 39, 976-81.
- Verheijen, F.W. et al. (1985) Eur J Biochem 149, 315-21.
- Rudenko, G. et al. (1998) Proc Natl Acad Sci USA 95, 621-5.
- Hohl, M. et al. (2020) J Biol Chem 295, 12605-12617.
- Kozlowski, L. et al. (2000) Arch Dermatol Res 292, 68-71.
- Ni, S. et al. (2018) Onco Targets Ther 11, 3835-3845.
- Wang, H. et al. (2021) Sci Rep 11, 14611.
- Hu, B. et al. (2020) Mol Med Rep 21, 2553-2559.
限制使用
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专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not for Use in Diagnostic Procedures.
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