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  3. PathScan® Bcr/Abl Activity Assay: Phospho-c-Abl, Phospho-Stat5 and Phospho-CrkL Multiplex Western Detection Kit

PathScan® Bcr/Abl Activity Assay: Phospho-c-Abl, Phospho-Stat5 and Phospho-CrkL Multiplex Western Detection Kit #7130

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    Product Information

    Product Description

    The Pathscan® Multiplex Western Detection Kit offers a unique method to assay the inhibition of multiple proteins on one membrane without stripping and reprobing. This method saves the user valuable time while increasing accuracy and minimizing reagent waste. The system allows the user to simultaneously detect the inhibition of phosphorylation of c-Abl, Stat5 and CrkL proteins in response to STI-571. The kit also includes eIF4E antibody to control protein loading. In addition, each Pathscan® Multiplex Western Detection Kit contains treated and untreated cell lysates and the Phototope®-HRP Western Detection System. The kit includes enough primary and secondary antibodies to perform five Western blot experiments.

    Specificity / Sensitivity

    Each phospho-antibody in this kit recognizes only the phosphorylated form of its specific target. The eIF4E control antibody detects total levels of eIF4E to determine protein loading. All of the antibodies detect endogenous levels of the target proteins.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with synthetic peptides. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    STI-571 (also known as Imatinib mesylate) is a tyrosine kinase (TK) inhibitor that is a relatively specific ATP-binding site antagonist of Bcr-Abl, PDGF receptor and c-Kit TKs (1-3). Results are encouraging in CML clinical trials, and STI-571 has become a paradigm for targeted cancer therapeutics (4-6). Signal transduction through phospho-tyrosine pathways has been studied extensively, and tyrosine phosphorylation has been linked to multiple cell growth and differentiation pathways (7-9). Because the observed leukemic state of CML is dependent on the intact Bcr-Abl tyrosine kinase activity, extensive work has been done to identify substrates of Bcr-Abl and thus possible mechanisms leading to a myeloid expansion. Many groups have characterized prominent tyrosine-phosphorylated protein substrates in both CML blasts and Bcr-Abl-expressing cell lines, including SHIP, c-cbl, Dok, SHC and CrkL (10-15). In addition, key signal transduction pathways involving PI3 kinase, Ras, Myc and Stat5 are also activated in a Bcr-Abl kinase-dependent manner (16).
    1. Buchdunger, E. et al. (1996) Cancer Res 56, 100-4.
    2. Heinrich, M.C. et al. (2000) Blood 96, 925-32.
    3. Druker, B.J. et al. (1996) Nat Med 2, 561-6.
    4. Mauro, M.J. and Druker, B.J. (2001) Curr Oncol Rep 3, 223-7.
    5. Druker, B.J. et al. (2001) N Engl J Med 344, 1031-7.
    6. Druker, B.J. et al. (2001) N Engl J Med 344, 1038-42.
    7. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
    8. Ullrich, A. and Schlessinger, J. (1990) Cell 61, 203-12.
    9. Cantley, L.C. et al. (1991) Cell 64, 281-302.
    10. ten Hoeve, J. et al. (1994) Blood 84, 1731-6.
    11. Matsuguchi, T. et al. (1994) J Biol Chem 269, 5016-21.
    12. Carpino, N. et al. (1997) Cell 88, 197-204.
    13. Sattler, M. et al. (1997) Oncogene 15, 2379-84.
    14. Di Cristofano, A. et al. (1998) J Biol Chem 273, 4827-30.
    15. Wisniewski, D. et al. (1999) Blood 93, 2707-20.
    16. Kabarowski, J.H. and Witte, O.N. (2000) Stem Cells 18, 399-408.

    Alternate Names

    stat5 a; stat5 b; stat5b; western cocktail

    Database Links

    UniProt ID: P62491 , Q15907 , P46109 , P42229 , P51692 , P00519


    Entrez-Gene Id: 8766 , 9230 , 1399 , 6776 , 6777 , 25


    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    For Research Use Only. Not for Use in Diagnostic Procedures.
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