Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ASC/TMS1 (D2W8U) Feline Chimeric mAb #96134
Filter:
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Feline Chimera IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as ASC/TMS1 (D2W8U) Rabbit mAb #67824.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ASC/TMS1 (D2W8U) Feline Chimeric mAb recognizes endogenous levels of total ASC/TMS1 protein.
Species Reactivity:
Mouse
Source / Purification
This recombinant chimeric antibody is engineered from ASC/TMS1 (D2W8U) Rabbit mAb #67824 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.
The parent antibody, ASC/TMS1 (D2W8U) Rabbit mAb #67824, is produced by immunizing animals with recombinant mouse ASC/TMS1 protein.
The parent antibody, ASC/TMS1 (D2W8U) Rabbit mAb #67824, is produced by immunizing animals with recombinant mouse ASC/TMS1 protein.
Background
TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22 kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The ASC/TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of ASC/TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis ASC/TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). ASC/TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).
- Masumoto, J. et al. (1999) J Biol Chem 274, 33835-8.
- Conway, K.E. et al. (2000) Cancer Res 60, 6236-42.
- Terasawa, K. et al. (2004) Clin Cancer Res 10, 2000-6.
- Stone, A.R. et al. (2004) Am J Pathol 165, 1151-61.
- Guan, X. et al. (2003) Int J Cancer 107, 202-8.
- Moriai, R. et al. (2002) Anticancer Res 22, 4163-8.
- Virmani, A. et al. (2003) Int J Cancer 106, 198-204.
- Das, P.M. et al. (2006) Mol Cancer 5, 28.
- Strong, R. et al. (1991) Brain Res 542, 23-8.
- Ohtsuka, T. et al. (2004) Nat Cell Biol 6, 121-8.
- Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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