Cat. # | Size | Price | Inventory |
---|---|---|---|
27987S | 100 µl |
REACTIVITY | M |
SENSITIVITY | Endogenous |
MW (kDa) | 30-50 |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Mouse
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Adora2b/Adenosine Receptor A2b protein.
Adenosine receptor A2b (A2B adenosine receptor, A2BAR, ADORA2B) belongs to the adenosine receptor group of G protein-coupled receptors (GPCRs) (1-6). The adenosine receptors are divided into A1, A2, and A3 subtypes based on their ability to inhibit or stimulate adenyl cyclase (1-3). Within the A2 subtype, the high-affinity receptor is designated the A2A adenosine receptor, and the low-affinity receptor is the A2B adenosine receptor (1-3). Adenosine receptor A2b has been identified as a regulator of inflammation and vascular adhesion, primarily via signals from hematopoietic cells to the vasculature (3). Adenosine receptor A2b couples to G proteins to activate adenyl cyclase, leading to increased intracellular cAMP levels, as well as activation of phospholipase C (PLC), leading to increased intracellular calcium (4-6). Multiple mediators associated with inflammation, such as LPS, TNF-α, and IFN-γ, have been demonstrated to increase A2BAR expression in certain cell types (4-6).
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