Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ADA2B (F6G5T) Rabbit mAb #10691
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ADA2B (F6G5T) Rabbit mAb recognizes endogenous levels of total ADA2B protein. This antibody does not cross-react with ADA2A protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp172 of human ADA2B protein.
Background
ADA2A and ADA2B are human homologs of the yeast SAGA complex member Ada2. While highly related, ADA2A and B appear functionally distinct. ADA2A is incorporated into PCAF-containing complexes, while ADA2B is recruited to SAGA complexes with GCN5 and Brg1 (1-3). ADA2 proteins form a trimer with GCN5L and ADA3, and enhance GCN5L acetyltransferase activity (4,5). The C-terminal domain of ADA2A and ADA2B determines incorporation into their various complexes, resulting in specific targeting toward histone H3 or H4 (6).
- Ogryzko, V.V. et al. (1998) Cell 94, 35-44.
- Barlev, N.A. et al. (2003) Mol Cell Biol 23, 6944-57.
- Kusch, T. et al. (2003) Mol Cell Biol 23, 3305-19.
- Horiuchi, J. et al. (1995) Mol Cell Biol 15, 1203-9.
- Gamper, A.M. et al. (2009) Mol Cell Biol 29, 266-80.
- Vamos, E.E. and Boros, I.M. (2012) FEBS Lett 586, 3279-86.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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