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α-Smooth Muscle Actin (1A4) Mouse mAb (BSA and Azide Free)

α-Smooth Muscle Actin (1A4) Mouse mAb (BSA and Azide Free) #69319

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Supporting Data

REACTIVITY	H M R Hm
SENSITIVITY	Endogenous
MW (kDa)	42
Source/Isotype	Mouse IgG2a

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster

Product Information

Product Usage Information

This product is the carrier free version of product #56856. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #56856.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

α-Smooth Muscle Actin (1A4) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total α-smooth muscle actin protein.

Species Reactivity:

Human, Mouse, Rat, Hamster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human α-smooth muscle protein.

Background

Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility (1). The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells (1).

Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.

Database Links

UniProt ID: P62736

Entrez-Gene Id: 59

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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