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  3. Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (InTraSeq™ 3' Conjugate 3078)
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Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (InTraSeq™ 3' Conjugate 3078) #31103

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  • SCA

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • SCA-Single Cell Analysis 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    Storage

    Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (InTraSeq™ 3' Conjugate 3078) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175.


    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.

    Background

    Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

    Database Links

    UniProt ID: P42574


    Entrez-Gene Id: 836


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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