ATF-2 Fusion Protein #9224
Inquiry Info. # 9224
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Product Specifications
| MW (kDa) | 34 |
Product Information
Product Description
Activating Transcription Factor 2 (ATF-2) Fusion Protein serves as a useful substrate for SAPK/JNK and p38 MAP kinases. It is expressed as a recombinant protein fusion containing ATF-2 residues 19-96. It contains the amino-terminal activation domain of ATF-2, which is regulated by phosphorylation of Thr69 and Thr71.
| MW (kDa) | 34 |
| Molecular Formula | Molecular Weight: 34 kDa |
Product Usage Information
ATF-2 Fusion Protein, at a concentration of 2 µg/20 µl reaction, can be phosphorylated by an upstream kinase in an in vitro kinase assay with 1X Kinase Buffer (#9802) and 200 µM ATP (#9804). After a 30-minute assay at 30ºC, phosphorylation can be detected by Western blot with Phospho-ATF-2 (Thr71) Antibody (#9221).
Storage
Store at -20°C.
Quality Control
The purified protein was identified by SDS-PAGE and western blot analysis, using ATF-2 Antibody #9222, to be greater than 90% ATF-2 Fusion Protein.
Source / Purification
Cloned from a human cDNA library (5) and overexpressed in E. coli.
Background
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
Alternate Names
ATF2; CRE-BP1; CREB2; pp110; substrate
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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