SignalKine™ Human LIF Sandwich ELISA Kit #11928
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Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
SignalKine™ Human LIF Sandwich ELISA Kit from Cell Signaling Technology (CST™) is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects human LIF (hLIF) in multiple matrices. Unknown samples being tested for hLIF and hLIF Standards are added to low volume microwells, where the hLIF is captured by the coated hLIF Rabbit mAb. Following a washing step, a biotinylated hLIF Rabbit mAb is added to detect the captured hLIF. HRP-linked Streptavidin is then used for detection of the biotinylated hLIF Detection Rabbit mAb. HRP substrate, TMB, is added for color development. The magnitude of absorbance for this developed color is proportional to the quantity of hLIF in the sample.
SignalKine™ Human LIF Sandwich ELISA Kit detects hLIF in multiple matrices that be quantified by generating a standard curve with the recombinant hLIF protein standard provided. The hLIF standard range is from 31.25 to 2000 pg/ml. Samples containing higher levels of hLIF can be diluted to fit into the standard range.
SignalKine™ Human LIF Sandwich ELISA Kit detects hLIF in multiple matrices that be quantified by generating a standard curve with the recombinant hLIF protein standard provided. The hLIF standard range is from 31.25 to 2000 pg/ml. Samples containing higher levels of hLIF can be diluted to fit into the standard range.
Specificity / Sensitivity
Species Reactivity:
Human
Background
Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise-induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not for Use in Diagnostic Procedures.
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