HTScan® PKD/PKCμ Kinase Assay Kit #7412
Inquiry Info. # 7412
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Product Information
Product Description
The kit provides a means of performing kinase activity assays with recombinant human PKD/PKCmu kinase. It includes active PKD/PKCmu kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate peptide.
| Molecular Formula | Peptide substrate, Biotin-peptide: 2,326 Daltons. GST-PKD/PKCmu Kinase domain: 133 kDa. |
Background
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).
- Nishizuka, Y. (1984) Nature 308, 693-698.
- Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
- Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
- Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
- Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
- Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
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