Cellular Glutathione Detection Assay Kit (#13859) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

UniProt ID:

#O15217

Entrez-Gene Id:

2941

Product Information

Storage

Upon receipt, Tris Assay Buffer (#13865) should be removed from #13859 and stored at 4°C. And remaining components should be stored at -20ºC.

Specificity / Sensitivity

The Cellular Glutathione Detection Assay Kit is expected to detect the reduced glutathione in cellular assays across all species.

Species Reactivity:

All Species Expected

Species predicted to react based on 100% sequence homology

All Species Expected

Product Description

The Cellular Glutathione Detection Assay Kit employs the cell permeable dye monochlorobimane (MCB) to detect reduced glutathione (GSH) in cellular assays. MCB displays a high affinity for reduced glutathione and exhibits a very low fluorescent yield when free in solution. Upon binding to GSH, the dye exhibits a strong blue fluorescence that can be measured at an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Fluorescent intensity correlates with sample GSH level. This kit can be used to either label cells directly or to detect GSH level in cell extracts. The assay can be easily applied in high throughput plate-format, flow cytometry, or fluorescent imaging.

Background

The antioxidant glutathione is found in both reduced and oxidized states in cells. Reduced glutathione can play an important role in preventing cellular damage caused by reactive oxygen species, including free radicals and peroxides. Reduced glutathione (GSH) acts as an electron donor in the presence of free radicals and peroxides to become oxidized (GSSG). GSH also participates in redox signaling through the removal of the cellular second messenger H₂O₂ (1,2). Diminished glutathione levels are observed during the aging process and in oxidative stress-related diseases. The depletion of GSH is necessary for the progression of apoptosis that is mediated by various signaling pathways (3,4). Intracellular GSH levels can be a very useful indicator for overall cell health, proliferation, and death (2).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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