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Thy1/CD90 (D3V8A) Rabbit mAb (Alexa Fluor® 647 Conjugate) #86662
Filter:
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Thy1/CD90 (D3V8A) Rabbit mAb #13801.
Fluorescent Properties
- ← Excitation: 650 nm ← Emission: 668 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Thy1/CD90 (D3V8A) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total Thy1/CD90 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val62 of human Thy1/CD90 protein.
Background
The Thy1/CD90 cell surface antigen is a GPI-anchored, developmentally regulated protein involved in signaling cascades that mediate neurite outgrowth, T cell activation, tumor suppression, apoptosis, and fibrosis (1). Thy1/CD90 is highly expressed on the surface of adult neurons and is thought to play a role in modulating adhesive and migratory events, such as neurite extension (1,2). Decreased Thy1/CD90 mRNA and protein expression is associated with the development of epithelial ovarian cancer, suggesting a role as a putative tumor suppressor gene of human ovarian cancer (3,4). Research studies indicate that Thy1/CD90 knockout mice have impaired cutaneous immune responses and abnormal retinal development (5,6). Thy1/CD90 is epigenetically regulated or deregulated in some disease states, such as pulmonary fibrosis. The potentially reversible hypermethylation of the Thy1/CD90 promoter offers the possibility of novel therapeutic options in this disease (7).
- Rege, T.A. and Hagood, J.S. (2006) FASEB J 20, 1045-54.
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- Abeysinghe, H.R. et al. (2003) Cancer Genet Cytogenet 143, 125-32.
- Zeng, L.Q. et al. (2009) Zhonghua Zhong Liu Za Zhi 31, 118-20.
- Beissert, S. et al. (1998) J Immunol 161, 5296-302.
- Simon, P.D. et al. (1999) Brain Res Dev Brain Res 117, 219-23.
- Sanders, Y.Y. et al. (2008) Am J Respir Cell Mol Biol 39, 610-8.
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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