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  3. STING (E9X7F) Rabbit mAb (SignalFlex™ mFluor™ Violet 510 Conjugate)
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STING (E9X7F) Rabbit mAb (SignalFlex™ mFluor™ Violet 510 Conjugate) #57837

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to mFluor™ Violet 510 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #90947

    Fluorescent Properties

    • ← Excitation: 412 nm ← Emission: 505 nm

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    STING (E9X7F) Rabbit mAb (SignalFlex™ mFluor™ Violet 510 Conjugate) recognizes endogenous levels of total STING protein. STING (E9X7F) Rabbit mAb (SignalFlex™ mFluor™ Violet 510 Conjugate) lacks sensitivity in some low-expressing mouse cell lines by immunofluorescence. This lack of sensitivity has not been observed in human cell lines.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human STING recombinant protein and reacts with an epitope near the carboxy terminus.

    Background

    Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7,8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).

    Database Links

    UniProt ID: Q86WV6


    Entrez-Gene Id: 340061


    限制使用

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    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    mFluor is a trademark of AAT Bioquest, Inc.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    mFluor is manufactured by AAT Bioquest, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.