Name: SMAD2/3 (D7G7) XP® Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate)
Price: 443.00 USD
Availability: InStock

Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Description

This Cell Signaling Technology^® antibody is conjugated to mFluor™ UV375 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #8685

Product Usage Information

SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology^® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

SMAD2/3 (D7G7) XP^® Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) recognizes endogenous levels of total SMAD2/3 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His198 of human SMAD2/3 protein.

Background

Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).

Database Links

UniProt ID: P84022 , Q15796

Entrez-Gene Id: 4088 , 4087

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

mFluor is a trademark of AAT Bioquest, Inc.

SignalFlex is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

mFluor is manufactured by AAT Bioquest, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

SMAD2/3 (D7G7) XP® Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) #92576