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Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate)

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (SignalFlex^™ mFluor^™ Red 780 Conjugate) #78597

Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Description

This Cell Signaling Technology^® antibody is conjugated to mFluor™ Red 780 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #4856

Fluorescent Properties

← Excitation: 629 nm ← Emission: 767 nm

Product Usage Information

SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology^® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate) detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).

Database Links

UniProt ID: P62753

Entrez-Gene Id: 6194

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

mFluor is a trademark of AAT Bioquest, Inc.

SignalFlex is a trademark of Cell Signaling Technology, Inc.

mFluor is manufactured by AAT Bioquest, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate) #78597