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NT5E/CD73 (D7F9A) Rabbit mAb (Alexa Fluor® 488 Conjugate) #14627
Filter:
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated NT5E/CD73 (D7F9A) Rabbit mAb #13160.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
NT5E/CD73 (D7F9A) Rabbit mAb (Alexa Flour® 488 Conjugate) recognizes endogenous levels of total NT5E/CD73 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.
Background
Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).
- Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
- Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
- Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
- Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
- Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
- Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
- Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
- Zylka, M.J. (2011) Trends Mol Med 17, 188-96.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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