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Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 532 Conjugate)

Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 532 Conjugate) #95241

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Mouse IgG2a kappa

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology^® antibody is conjugated to Alexa Fluor^® 532 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Myc-Tag (9B11) Mouse mAb #2276.

Product Usage Information

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 532 Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 532 Conjugate) detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.
The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin ~90kDa.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems (1).

Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

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Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 532 Conjugate) #95241