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LT-beta-R (E5I6X) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) #81130
Filter:
- F
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated LTβR (E5I6X) Rabbit mAb #57560.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
LT-beta-R (E5I6X) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total LTβR protein. This antibody conjugate is expected to recognize multiple isoforms of LTβR protein. This antibody conjugate cross-reacts with an unidentified protein of 20 kDa and 30 kDa in some cell extracts.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg428 of human LTβR protein.
Background
Lymphotoxin β receptor (LTβR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3) and tumor necrosis factor receptor type III (TNF-RIII), is a type I membrane receptor expressed by stromal cells, including endothelial, mesenchymal, and epithelial cells, and by myeloid cells, such as dendritic cells and macrophages (1-4). When lymphotoxin α (LTα) is co-expressed with lymphotoxin β (LTβ) that possesses a transmembrane domain, it forms a cell surface-bound heterotrimer (LTα1β2) that exclusively binds to LTβR (5,6). The LTα1β2/LTβR pathway is required for the normal development of lymph nodes and Peyer’s patches (7,8). Disruption of the LTα1β2/LTβR pathway results in impaired protection from viral and bacterial infections (9,10). LTβR is also a receptor for LIGHT/TNFSF14 (11,12). LTβR signaling through LIGHT/TNFSF14 regulates self-renewal and differentiation of hematopoietic and leukemic stem cells (13). In cancer cells, LTβR signaling via TRAF molecules promotes apoptosis (14-16). Agonistic antibodies to LTβR trigger cancer cell death and suppress tumor growth in vivo (17).
- Murphy, M. et al. (1998) Cell Death Differ 5, 497-505.
- Browning, J.L. and French, L.E. (2002) J Immunol 168, 5079-87.
- Browning, J.L. et al. (1997) J Immunol 159, 3288-98.
- Kabashima, K. et al. (2005) Immunity 22, 439-50.
- Androlewicz, M.J. et al. (1992) J Biol Chem 267, 2542-7.
- Browning, J.L. et al. (1993) Cell 72, 847-56.
- De Togni, P. et al. (1994) Science 264, 703-7.
- Fütterer, A. et al. (1998) Immunity 9, 59-70.
- Ehlers, S. et al. (2003) J Immunol 170, 5210-8.
- Wege, A.K. et al. (2014) Innate Immun 20, 461-70.
- Maeda, T. et al. (2018) J Immunol 201, 202-214.
- Schneider, K. et al. (2004) Immunol Rev 202, 49-66.
- Höpner, S.S. et al. (2021) Nat Commun 12, 1065.
- Browning, J.L. et al. (1996) J Exp Med 183, 867-78.
- Force, W.R. et al. (1997) J Biol Chem 272, 30835-40.
- Rooney, I.A. et al. (2000) J Biol Chem 275, 14307-15.
- Lukashev, M. et al. (2006) Cancer Res 66, 9617-24.
Alternate Names
CD18; D12S370; LT-BETA-R; LTBR; lymphotoxin B receptor; lymphotoxin beta receptor; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; TNF-R-III; TNF-RIII; TNFCR; TNFR superfamily, member 3; TNFR-III; TNFR-RP; TNFR2-RP; TNFR3; TNFRSF3; TNR3; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; Tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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