GATA-4 (D3A3M) Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) #74433
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to mFluor™ UV375 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #36966
Product Usage Information
SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.
Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
Storage
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
GATA-4 is crucial for cardiomyocyte differentiation (4), and not surprisingly, mutations in the GATA-4 gene are implicated in many cardiac diseases, such as tetralogy of Fallot (5), familial and sporadic dilated cardiomyopathy (6,7), and atrial septal defect (8). GATA-4 and GATA-6 together maintain intestinal epithelial structure by regulating enterocyte gene expression (9). They also have overlapping roles in steroidogenesis and genital ridge formation during gonadal development (10). GATA-4 is also a required factor for bone mineralization in osteoblast progenitor cells, where it suppresses TGF-β and activates BMP signaling (11). Research studies have uncovered crosstalk among the transcription factors GATA-4, GATA-6, and KLF5 that promotes gastric cancer development (12). Additional studies describe the role of histone H3K9 hyperacetylation on the GATA-4 promoter in fetal mice that have been exposed to ethanol (13).
- Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
- Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
- Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
- Gan, L. et al. (2014) Gene Expr Patterns 16, 8-22.
- Yang, Y.Q. et al. (2013) Hum Mutat 34, 1662-71.
- Li, R.G. et al. (2013) Biochem Biophys Res Commun 439, 591-6.
- Li, J. et al. (2014) Gene 548, 174-81.
- Mohan, R.A. et al. (2014) Am J Med Genet A 164A, 2732-8.
- Walker, E.M. et al. (2014) Dev Biol 392, 283-94.
- LaVoie, H.A. (2014) Biol Reprod 91, 38.
- Güemes, M. et al. (2014) J Bone Miner Res 29, 2676-87.
- Chia, N.Y. et al. (2015) Gut 64, 707-19.
- Peng, C. et al. (2014) PLoS One 9, e104135.
限制使用
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