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CD68 (E3O7V) Rabbit mAb (SignalFlex mFluor UV375 Conjugate) #46586

    Supporting Data

    REACTIVITY M R Hm
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 
    • Hm-Hamster 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to mFluor™ UV375 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #97778

    Fluorescent Properties

    • ← Excitation: 351 nm ← Emission: 387 nm

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    CD68 (E3O7V) Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

    Species Reactivity:

    Mouse, Rat, Hamster

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

    Background

    CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    mFluor is a trademark of AAT Bioquest, Inc.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    mFluor is manufactured by AAT Bioquest, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.