R Recombinant
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CD206/MRC1 (E6T5J) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #36508
Filter:
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated CD206/MRC1 (E6T5J) XP® Rabbit mAb #24595.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD206/MRC1 (E6T5J) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total CD206/MRC1 protein. This antibody recognizes mouse CD206/MRC1 protein and is also reactive with human CD206/MRC1; however, this antibody is not suggested for immunohistochemical analysis of human tissues. Instead, CD206/MRC1 (E2L9N) Rabbit mAb #91992 is recommended for IHC analysis of human tissue samples.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD206/MRC1 protein.
Background
The mannose receptor (MR/CLEC13D/CD206/MMR/MRC1/Macrophage mannose receptor 1) is an endocytic receptor expressed by populations of dendritic cells, macrophages, and nonvascular endothelium (1). The mannose receptor is a heavily glycosylated type I transmembrane protein with three types of extracellular domains and a short carboxy-terminal cytoplasmic domain with no apparent signaling motif (2-4). The extracellular portion of the protein is made up of a CR domain, which binds sulfated glycans, an FNII domain, which binds collagens, and eight C-type lectin domains, which bind carbohydrates containing mannose, fucose, or GlcNAc (4-7). The receptor recycles between the plasma membrane and early endosomes (8). Functions include a role in antigen cross-presentation, clearance of endogenous proteins, pathogen detection, and trafficking through lymphatic vessels (9-12).
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- Lennartz, M.R. et al. (1989) J Biol Chem 264, 2385-90.
- Wileman, T.E. et al. (1986) Proc Natl Acad Sci U S A 83, 2501-5.
- Taylor, M.E. et al. (1990) J Biol Chem 265, 12156-62.
- Fiete, D.J. et al. (1998) Proc Natl Acad Sci U S A 95, 2089-93.
- Napper, C.E. et al. (2006) Biochem J 395, 579-86.
- Fiete, D. et al. (1997) Proc Natl Acad Sci U S A 94, 11256-61.
- Tietze, C. et al. (1982) J Cell Biol 92, 417-24.
- Burgdorf, S. et al. (2006) J Immunol 176, 6770-6.
- Lee, S.J. et al. (2002) Science 295, 1898-901.
- Milone, M.C. and Fitzgerald-Bocarsly, P. (1998) J Immunol 161, 2391-9.
- Marttila-Ichihara, F. et al. (2008) Blood 112, 64-72.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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