CD14 (61D3) Mouse mAb (PerCP-Cy5.5® Conjugate) #99811
Filter:
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometry analysis in human cells.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:20 |
| Flow Cytometry (Live) | 1:20 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4oC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD14 (61D3) Mouse mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total CD14 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5).
The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage (6).
The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage (6).
- Wright, S.D. et al. (1991) J Exp Med 173, 1281-6.
- Ziegler-Heitbrock, H.W. and Ulevitch, R.J. (1993) Immunol Today 14, 121-5.
- Suzuki, M. et al. (2009) J Immunol 182, 6485-93.
- Pugin, J. et al. (1994) Immunity 1, 509-16.
- Zanoni, I. et al. (2011) Cell 147, 868-80.
- Flora, P.K. and Gregory, C.D. (1994) Eur J Immunol 24, 2625-32.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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