CD11b/ITGAM (M1/70) Rat mAb (PE-Cy5® Conjugate) #16538
Filter:
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rat IgG2b kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PE-Cy5® and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:160 |
| Flow Cytometry (Live) | 1:160 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD11b/ITGAM (M1/70) Rat mAb (PE-Cy5® Conjugate) recognizes endogenous levels of total CD11b protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human, Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).
The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6).
The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6).
- Solovjov, D.A. et al. (2005) J Biol Chem 280, 1336-45.
- Murray, P.J. and Wynn, T.A. (2011) Nat Rev Immunol 11, 723-37.
- Fagerholm, S.C. et al. (2006) Blood 108, 3379-86.
- Rosetti, F. and Mayadas, T.N. (2016) Immunol Rev 269, 175-93.
- Finkelman, F.D. et al. (1996) J Immunol 157, 1406-14.
- Holmberg, L.A. et al. (1981) J Immunol 127, 1792-9.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
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