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CD11b/ITGAM (M1/70) Rat Monoclonal Antibody (APC Conjugate)

CD11b/ITGAM (M1/70) Rat Monoclonal Antibody (APC Conjugate) #41249

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Product Specifications

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rat IgG2b kappa

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:160
Flow Cytometry (Live)	1:160

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD11b/ITGAM (M1/70) Rat Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total CD11b protein. This antibody conjugate detects an epitope within the extracellular domain.

Species Reactivity:

Human, Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

Cluster of differentiation molecule 11b (CD11b)/Integrin alpha M (ITGAM) is a transmembrane protein forming heterodimers that are composed of α and β subunits (1). CD11b is expressed by, and commonly used as a marker for, myeloid lineage cells, including neutrophils, monocytes, macrophages, and microglia (2). CD11b is phosphorylated at Ser1126 (cytoplasmic tail) in neutrophils. Research has shown that this phosphorylation event plays a role for leukocytes traveling from the bloodstream to tissues (3). Furthermore, genome-wide association studies have linked CD11b to autoimmune diseases, such as systemic lupus erythematosus (SLE) (4).

The M1/70 antibody is widely used as a marker for CD11b expression on neutrophils, macrophages, NK cells, and dendritic cells (DC) (5,6).

Alternate Names

antigen CD11b (p170); CD11 antigen-like family member B; CD11B; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; Integrin alpha-M; integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM; ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6

Database Links

UniProt ID: P11215

Entrez-Gene Id: 3684

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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