Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
BLK (E8T1B) Rabbit mAb (Biotinylated) #71051
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is the biotinylated version of the unconjugated BLK (E8T1B) Rabbit mAb #66002 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 μg/mL. Peptide ELISA data were generated using the biotinylated antibody.
| MW (kDa) | 55 |
Product Usage Information
Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.
Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.
Storage
Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
BLK (E8T1B) Rabbit mAb (Biotinylated) recognizes endogenous levels of total BLK protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro228 of human BLK protein.
Background
BLK is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of BLK (3). It has been suggested that BLK is involved in the control of B cell differentiation and proliferation (4,5). BLK transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that BLK may play an important role in thymopoiesis (6). BLK function may be redundant, however, as mice that do not express BLK are not impaired with respect to B cell development and immune response (7).
- Dymecki, S.M. et al. (1990) Science 247, 332-336.
- Wasserman, R. et al. (1995) J. Immunol. 155, 644-651.
- Sefton, B.M. et al. (1994) Curr. Opin. Immunol. 6, 372-379.
- Malek, S.N. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 7351-7356.
- Yao, X.R. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 7946-7950.
- Islam, K.B. et al. (1995) J. Immunol. 154, 1265-1272.
- Texido, G. et al. (2000) Mol Cell Biol 20, 1227-33.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not for Use in Diagnostic Procedures.
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