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BiP (C50B12) Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate)

BiP (C50B12) Rabbit mAb (SignalFlex^™ mFluor^™ UV375 Conjugate) #77376

Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Description

This Cell Signaling Technology^® antibody is conjugated to mFluor™ UV375 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #3177

Fluorescent Properties

← Excitation: 351 nm ← Emission: 387 nm

Product Usage Information

SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology^® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

BiP (C50B12) Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) detects endogenous levels of total BiP protein.

Species Reactivity:

Human, Mouse

Source / Purification

BiP (C50B12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gly584 of human BiP.

Background

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).

Database Links

UniProt ID: P11021

Entrez-Gene Id: 3309

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not for Use in Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

mFluor is a trademark of AAT Bioquest, Inc.

SignalFlex is a trademark of Cell Signaling Technology, Inc.

mFluor is manufactured by AAT Bioquest, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

BiP (C50B12) Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) #77376