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4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (SignalFlex mFluor Red 780 Conjugate) #18101

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to mFluor™ Red 780 fluorescent dye under optimal conditions and formulated at 200 µg/mL. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated #34594

    Product Usage Information

    SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.

    Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Specificity / Sensitivity

    4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate) recognizes endogenous levels of total 4-1BB/CD137/TNFRSF9 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the extracellular domain of human 4-1BB/CD137/TNFRSF9 protein.

    Background

    TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1,2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6).
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    mFluor is a trademark of AAT Bioquest, Inc.
    SignalFlex is a trademark of Cell Signaling Technology, Inc.
    mFluor is manufactured by AAT Bioquest, Inc.
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