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Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100)

Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) #5020

Inquiry Info. # 5020

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Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Source/Isotype	Mouse

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) is a high affinity antibody that can be used in high throughput kinase assays and drug discovery applications. Results obtained from ELISA assays against a wide variety of phosphopeptides indicate that (a) P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence, and (b) P-Tyr-100 binds to a larger number of phospho-Tyr-containing peptides than any other commercially available anti-phospho-Tyr monoclonal antibody, including 4G10 and PY20. This antibody does not cross-react with peptides containing phospho-Ser or phospho-Thr. (Patented, U.S. No. 6,441,140 and Patents Pending.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine-containing peptides .

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
Description: This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is
a sample antibody and intended for use by drug discovery scientists.
Please direct inquiries for bulk pricing of this antibody, peptide substrates, or protocols to [email protected].

Alternate Names

4g10; anti-phosphotyrosine; general; HTS; HTS antibody; motif; PBS; PBS only; phospho-tyrosine; phosphotyrosine; screening; tyrosine; tyrosine kinase assay

限制使用

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For Research Use Only. Not for Use in Diagnostic Procedures.

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