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  3. Pim-2 Antibody

Pim-2 Antibody #4723

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Product Name

4730 Pim-2 (D1D2) Rabbit mAb
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  • WB

Inquiry Info. # 4723

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40, 38, 34
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Pim-2 Antibody detects endogenous levels of total Pim-2 protein. The antibody does not cross-react with other Pim family members.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln287 of human Pim-2. Antibodies were purified by peptide affinity chromatography.

    Background

    Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).
    Pim-2 is highly homologous to Pim-1 with similar oncogenic functions (13,14). Three isoforms of Pim-2 can be generated from alternative start sites which run at 34, 38, and 40 kDa (13). Pim-2 leads to resistance to a variety of apoptotic stimuli and its expression is negatively regulated by growth factor withdrawal (15,16). Increased levels of Pim-2 has also been observed in certain cancers (17,18).
    1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
    2. Selten, G. et al. (1986) Cell 46, 603-11.
    3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
    4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
    5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
    6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
    7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
    8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
    9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
    10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
    11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
    12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
    13. van der Lugt, N.M. et al. (1995) EMBO J. 14, 2536-2544.
    14. Breuer, M.L. et al. (1989) EMBO J. 8, 743-748.
    15. Fox, C.J. et al. (2003) Genes Dev. 17, 1841-1854.
    16. White, E. (2003) Genes Dev. 17, 1813-1816.
    17. Cohen, A.M. et al. (2004) Leuk. Lymphoma 45, 951-955.
    18. Dai, H. et al. (2005) Prostate 65, 276-286.

    Database Links

    UniProt ID: Q9P1W9


    Entrez-Gene Id: 11040


    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

    专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专

    For Research Use Only. Not for Use in Diagnostic Procedures.
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