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CR1/CD35 (E8B1Z) Rabbit mAb (Alexa Fluor® 647 Conjugate) #17519
Filter:
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated CR1/CD35 (E8B1Z) Rabbit mAb #96615.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CR1/CD35 (E8B1Z) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total CR1/CD35 protein. Non-specific staining was observed in skeletal muscle by immunohistochemistry.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CR1/CD35 protein.
Background
Complement receptor type 1 (CR1/CD35) is a type I transmembrane glycoprotein that is expressed on the surface of B cells, neutrophils, monocytes, and renal podocytes (1,2). As a component of the host innate immune system, CR1/CD35 expressed on neutrophils and monocytes binds to ligands coated with the complement opsonins, C3b and C4b, which facilitates phagocytosis and production of proinflammatory cytokines (3,4). CR1/CD35 also participates in negative regulation of the complement cascade through its ability to promote dissociation of C3 and C5 convertases (4,5) and by serving as one of multiple cofactors for factor-I-mediated cleavage and inactivation of C3b and C4b (6).
- Fearon, D.T. (1980) J Exp Med 152, 20-30.
- Tedder, T.F. et al. (1983) J Immunol 130, 1668-73.
- Krych-Goldberg, M. and Atkinson, J.P. (2001) Immunol Rev 180, 112-22.
- Bacle, F. et al. (1990) J Immunol 144, 147-52.
- Hourcade, D.E. et al. (2002) J Biol Chem 277, 1107-12.
- Masaki, T. et al. (1992) J Biochem 111, 573-8.
限制使用
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For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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