SignalSilence® GSK-3α/β siRNA (#6301) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

UniProt ID:

#P49840, #P49841

Entrez-Gene Id:

2931, 2932

Product Information

Product Usage Information

CST recommends transfection with 50 nM GSK-3alpha/beta siRNA 48 hours prior to cell lysis. See Protocol for transfection procedure.

Storage

GSK-3α/β siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Specificity / Sensitivity

GSK-3α/β siRNA will inhibit human, mouse and rat GSK-3α/β expression.

Species Reactivity:

Human

Species predicted to react based on 100% sequence homology

Mouse, Rat

Product Description

SignalSilence^® GSK-3α/β siRNA from Cell Signaling Technology allows the researcher to specifically inhibit GSK-3aα and GSK-3bβ expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
Small Interfering RNA (siRNA) has been used to specifically silence GSK-3alpha and beta expression, causing an upregulation of beta-catenin protein levels (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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