#P00778
Product Information
Product Usage Information
PTMScan® Wild Type Alpha-Lytic Protease (WaLP) is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the initial protein digestion step. Samples in urea lysis buffer need to be diluted to 2M or less urea prior to addition of WaLP. Dilute the 0.4 mg/ml stock 1:100 for final digest and incubate overnight at 37°C. Aliquot and store at -20ºC. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.
Storage
Specificity / Sensitivity
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
All Species Expected
Source / Purification
WaLP was produced recombinantly in E. coli, purified and is provided in 10 mM sodium acetate pH 5.0.
Product Description
Background
Wild type alpha-lytic protease (WaLP) was first isolated from myxobacterium Lysobacter enzymogenes (1). It is a protease that cleaves after A, S, T, and V residues making it a useful alternative protease for proteomics use. The pro-form of the enzyme is expressed as a 397 amino acid protein which is cleaved to a 198 amino acid mature protein. Numerous studies have been published about the mature form, active site, and catalytic mechanism (2, 3, 4). Based on crystal structure studies there seems to be a strong relation to other pancreatic serine proteases (5). The alternative cleavage specificity of WaLP is especially useful for looking at Proteomics of SUMO modifications as it cleaves the SUMO protein leaving a di-glycine motif that can then be detected using the proprietary SUMO Branch Motif remnant (K-ε-GG) antibody (6).
- Whitaker, DR. The alpha-Lytic Protease. Methods in Enzymology, 19, 599-613
- Sohl, JL, et al., , IntramolecularChaperones and Protein Folding, Landes Company, Austin TX (1995) ISBN 978-1570592928 pp. 61-83
- Bauer, C.A. et al. (1981) Eur J Biochem 120, 289-94.
- Bachovchin, W.W. et al. (1981) Proc Natl Acad Sci U S A 78, 7323-6.
- Olson, M.O. et al. (1970) Nature 228, 438-42.
- Meyer, J.G. et al. (2014) Mol Cell Proteomics 13, 823-35.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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