WB, IP
H M R Mk
Endogenous
18
Rabbit IgG
#P68036
7332
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Xenopus, Zebrafish, Bovine, Dog, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu125 of human UBE2L3 protein.
Background
Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).
UBE2L3, also commonly referred to as UBCH7, is a ubiquitin-conjugating enzyme that belongs to a family of four related human genes, UBE2L1-UBE2L4. It appears as though UBE2L3 is the only member of this gene family to be transcribed and translated and that UBE2L1, UBE2L2, and UBE2L4 exist as pseudogenes (2,3). As a ubiquitin-conjugating enzyme, UBE2L3 has been linked to the ubiquitination of numerous substrates via its interaction with protein-ubiquitin E3 ligases such as NEDD4 (4), E6AP (5), Parkin (6), c-Cbl (7), and Triad1 (8,9). There is also evidence suggesting that UBE2L3 can modulate the transcriptional activity of numerous members of the nuclear hormone receptor superfamily such as the glucocorticoid receptor, progesterone receptor, androgen receptor, and retinoic acid receptors (10). Furthermore, UBE2L3 protein levels appear to be regulated by the ubiquitin-proteasome pathway allowing for it to exert control over S-phase entry and progression (11).
- Hershko, A. (1988) J Biol Chem 263, 15237-40.
- Ardley, H.C. et al. (2000) Biochim Biophys Acta 1491, 57-64.
- Moynihan, T.P. et al. (1996) Mamm Genome 7, 520-5.
- Anan, T. et al. (1998) Genes Cells 3, 751-63.
- Huang, L. et al. (1999) Science 286, 1321-6.
- Shimura, H. et al. (2001) Science 293, 263-9.
- Yokouchi, M. et al. (1999) J Biol Chem 274, 31707-12.
- Marteijn, J.A. et al. (2009) Leukemia 23, 1480-9.
- Marteijn, J.A. et al. (2005) Blood 106, 4114-23.
- Verma, S. et al. (2004) Mol Cell Biol 24, 8716-26.
- Whitcomb, E.A. et al. (2009) Mol Biol Cell 20, 1-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
限制使用
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