WB, IP
H
Endogenous
80
Rabbit IgG
#Q13214
7869
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln717 of human semaphorin 3B protein.
Background
Semaphorins are a family of cell surface and secreted proteins initially recognized as axon guidance factors that control the development of the central nervous system (1). They are involved in cell migration, angiogenesis, and immune responses (2-6). Based on protein structure, there are eight classes of semaphorins. Class 3-7 semaphorins are expressed in vertebrates. Semaphorin 3 subfamily members are the only secreted semaphorins in vertebrates. There are seven semaphorin 3 proteins and their receptors include neuropilins and the type A/D family plexins (7-9).
Semaphorin 3B functions as a tumor suppressor, as research studies have shown that it is deleted or inactivated in lung and breast cancer (10,11). Overexpression of semaphorin 3B inhibits tumor cell proliferation and causes apoptosis (12,13). Semaphorin 3B also inhibits angiogenesis (14). Semaphorin 3B loses its activity upon cleavage by furin-like pro-protein convertases (14).
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- Kruger, R.P. et al. (2005) Nat Rev Mol Cell Biol 6, 789-800.
- Staton, C.A. (2011) Biochem Soc Trans 39, 1565-70.
- Ghanem, R.C. et al. (2011) Curr Eye Res 36, 989-96.
- Nakagawa, Y. et al. (2011) J Immunol 186, 2881-8.
- Suzuki, K. et al. (2008) Nat Immunol 9, 17-23.
- He, Z. and Tessier-Lavigne, M. (1997) Cell 90, 739-51.
- Kolodkin, A.L. et al. (1997) Cell 90, 753-62.
- Chen, H. et al. (1997) Neuron 19, 547-59.
- Tomizawa, Y. et al. (2001) Proc Natl Acad Sci U S A 98, 13954-9.
- Nasarre, P. et al. (2005) Med Sci (Paris) 21, 641-7.
- Tse, C. et al. (2002) Cancer Res 62, 542-6.
- Castro-Rivera, E. et al. (2004) Proc Natl Acad Sci U S A 101, 11432-7.
- Varshavsky, A. et al. (2008) Cancer Res 68, 6922-31.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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